the effect of some flavonoids on in-vitro non-enzymatic glycosylation of proteins

introduction: non-enzymatic glycosylation of proteins which is the major cause of diabetic complications, cardiovascular diseases retinopathy, nephropathy and neuropathy can effectively be inhibted by antioxidants. materials and method: the antioxidant effects of several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin a were studied  on glycosylation of insulin, hemoglobin and albuminin in-vitro . optimal glucose concentration as well as incubation time were determined and inhibition percentage of the proteins were measured at three different concentrations (0.5,5 and 10 μg/ml) using spectrophotometric method. results: this study showed that biochanin a has potent antioxicant effects as it inhibits glycosylation of hemoglobin insulin and albumin by 100%, 60% and 100% respectively.apigenin is also inhibits albumin glycosylation by 100%. conclusion: as potent antioxidant effects are induced by flavenoedes, preventive effects of some plants containing the flavonoids on diabetic complication is expected.